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We show that FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), a recently identified plant-specific ESCRT component essential for multivesicular body biogenesis, plays...
Here, we provide the mechanism underlying a plant unique protein FREE1, upon autophagy-induced SnRK1α1-mediated phosphorylation, functions as a linkage between ATG conjugation system and ESCRT ...
The plant‐specific FYVE domain‐containing protein FREE1 acts as a cofactor of microprocessor through interacting with CPL1 in the nucleus. This interaction regulates the phosphorylation status of H...
FREE1 binds to phosphatidylinositol-3-phosphate (PI3P) and ubiquitin and specifically interacts with Vps23 via PTAP-like tetrapeptide motifs to be incorporated into the ESCRT-I complex. Arabidopsis free1 mutant is seedling lethal and defective in the formation of ILVs in MVBs.
FREE1 is a plant-specific and PVC-localized FYVE domain protein binding to PI3P. •. FREE1 is in a complex with ESCRT-I via a direct interaction with Vps23. •. FREE1 directly binds to ubiquitin and regulates vacuolar membrane protein sorting. •. FREE1 is essential for PVC/MVB biogenesis and plant growth. Summary.
FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which was previously identified as a plant-specific ESCRT component, modulates MVB-mediated endosomal sorting and autophagic degradation. Although the basic cellular functions of FREE1 as an ESCRT component have been described, the regulators that control FREE1 turnover remain unknown.
Here, we report the characterization of RESURRECTION1 (RST1) as a suppressor of free1 that, when mutated as a null mutant, restores the normal MVB and vacuole formation of a FREE1-RNAi knockdown line and consequently allows survival.
The vacuolar transport of ubiquitinated FREE1 protein is mediated by the autophagy pathway. As a consequence, the autophagy deficient mutants, atg5-1 and atg7-2, accumulate more endogenous FREE1 protein and display hypersensitivity to iron deficiency.
A plant-unique ESCRT component FREE1 mediates lipid droplet degradation in germinating seedlings by interacting with PEX11e and SDP1 to regulate peroxisoma
The FYVE domain protein required for endosomal sorting 1 (FREE1), which was previously identified as a plant-specific component of the endosomal sorting complex required for transport machinery, plays an essential role in endosomal trafficking.