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A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.
A real-time polymerase chain reaction ( real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.
Multiplex ligation-dependent probe amplification. Multiplex ligation-dependent probe amplification ( MLPA) is a variation of the multiplex polymerase chain reaction that permits amplification of multiple targets with only a single primer pair. [ 1] It detects copy number changes at the molecular level, and software programs are used for analysis.
Users. 30 million (as of 2024) G2A.COM Limited (commonly referred to as G2A) is a digital marketplace headquartered in the Netherlands, [ 1][ 2] with offices in Poland and Hong Kong. [ 3][ 4] The site operates in the resale of gaming offers and others digital items by the use of redemption keys. G2A.COM’s main offerings are game key codes for ...
DNA sequencer. A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G ( guanine ), C ( cytosine ), A ( adenine) and T ( thymine ). This is then reported as a text string, called a read.
Thermal cycler. The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). [ 1] Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme ...
Massive parallel sequencing. Massive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing ( NGS) or second-generation sequencing. Some of these technologies emerged between 1993 and 1998 ...
DNA replication. DNA Polymerase I (PDB) Molecular mechanism of PCR. A strip of eight PCR tubes. The history of the polymerase chain reaction (PCR) has variously been described as a classic "Eureka!" moment, [ 1] or as an example of cooperative teamwork between disparate researchers. [ 2] Following is a list of events before, during, and after ...